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URL: http://www.addgene.org/124504
Proper Citation: RRID:Addgene_124504
Insert Name: Tmem98
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:31961879
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector). To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted. The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
