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URL: http://www.addgene.org/11944
Proper Citation: RRID:Addgene_11944
Insert Name: 14-3-3 sigma
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9428519
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK?; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org