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URL: http://www.addgene.org/117117
Proper Citation: RRID:Addgene_117117
Bacterial Resistance: Kanamycin
Defining Citation: PMID:31147909
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pET-Gate2; Vector Types:Backbone for Golden gate assembly for gene targeting in Bacillus subtilis; Bacterial Resistance:Kanamycin
Comments: Backbone vector for reconstitution of gene targeting constructs by Golden gate assembly. The MazEF toxin-antitoxin system couter-selects for single cross-over events that leads to incorporation of the vector backbone into the genome. Overhangs generated upon BsaI cleavage : CGAG (RU) and CCAT (RD). (Gruber lab reference pSG1525)
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
