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URL: http://www.addgene.org/11237
Proper Citation: RRID:Addgene_11237
Insert Name: IRS-1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10958681
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: IRS-1 pBABE was cut with ApaLI and SalI. A 5' IRS-1 oligo at bp4746 (amino acid 1242) and 3'IRS-1 oligo with 3' IRS-1 sequence fused to the p21ras CAAX prenylation motif (CMSCKCVLS) were used for PCR and the insert was ligated into IRS-I pBABE. (Kahn lab plasmid name = IRS-1 CAAX (Human) pBABE)
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Source: Addgene
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