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URL: http://www.addgene.org/110949
Proper Citation: RRID:Addgene_110949
Insert Name: ccd B cassette
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:29448102
Vector Backbone Description: Backbone Marker:Yingfeng An; Vector Backbone:pANY1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: During molecular cloning, DNA of interest can be inserted into pANY1 using both MCS-1 and MCS-2, with deletion of the ccdB cassette. Thus the recombinant plasmid can be transformed into commonly used E. coli strains (e.g., JM109, BL21, DH5a). Because the original plasmid, pANY1, cannot be transformed into these E. coli strains, false positives can be eliminated.
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Source: Addgene
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