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URL: http://www.addgene.org/110940
Proper Citation: RRID:Addgene_110940
Insert Name: shRFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28396387
Vector Backbone Description: Backbone Marker:Dmitri Wiederschain; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: Recombination can be minimized by using NEB Stable E. coli strain grown at 30C to amplify the plasmid DNA. Even so, low recombination rate may still happen and when you eventually amplify the DNA you may need to screen through several colonies and perform diagnostic digests to identify the correct ones.
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Source: Addgene
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