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URL: http://www.addgene.org/110625
Proper Citation: RRID:Addgene_110625
Insert Name: St1Cas9 LMD-9
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31900288
Vector Backbone Description: Vector Backbone:MSP1594 (Plasmid #65775); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: This plasmid is identical to MSP1594 (Plasmid #65775) except for the addition of an SV40 NLS to the N-terminus of mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
