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URL: http://www.addgene.org/110624
Proper Citation: RRID:Addgene_110624
Insert Name: St1Cas9 LMD-9
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31900288
Vector Backbone Description: Vector Backbone:pX602 (Plasmid #61593); Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Comments: Mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9 was subcloned from MSP1594 (Plasmid #65775). An SV40 NLS was added to the N-terminus. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
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No rating or validation information has been found for pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
