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URL: http://www.addgene.org/11016
Proper Citation: RRID:Addgene_11016
Insert Name: EGFR D770-N771 insNPG
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16187797
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Mutation made by using the following primers to PCR from plasmid 11011: 5'-GGCCAGCGTGGACAACCCCGGCAACCCCCACGT-3' and 5'-ACGTGGGGGTTGCCGGGGTTGTCCACGCTGGCC-3'
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org