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URL: http://www.addgene.org/109288
Proper Citation: RRID:Addgene_109288
Insert Name: Multiple Cloning Site and GFP
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22820329
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: GFP cloned into pSUM36 in reverse orientation so that it is expressed off the 3' end of the kanamycin cassette.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org