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URL: http://www.addgene.org/108322
Proper Citation: RRID:Addgene_108322
Insert Name: Bxb1 attB site
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:30538179
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Comments: ORBIT integrating plasmid for creating knockouts of target genes. Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
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No rating or validation information has been found for pKM464 (deletions).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
