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URL: http://www.addgene.org/107690
Proper Citation: RRID:Addgene_107690
Insert Name: MAPRE1
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:29379139
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: LOV2 domain starts with LAAA reported to stabilize Jα helix docking (Strickland et al. PMID: 22388287) and mutations I427V and H519L resulting in faster dark state recovery. This LOV2 domain has the following mutations: I427V, H519L. The I427V mutation increases the dark state reversion rate of the LOV2 domain. Zdk1 binds efficiently to this LOV2 domain.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
