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URL: http://www.addgene.org/105866
Proper Citation: RRID:Addgene_105866
Insert Name: U6-26p::sgRNA scaffold
Organism: Arabidopsis thaliana
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34541122
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pGEM T-easy; Vector Types:Plant Expression, CRISPR, Synthetic Biology, Subcloning; Bacterial Resistance:Ampicillin
Comments: The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%. For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( http://www.bio-protocol.org/e2384 ).
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Source: Addgene
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