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URL: http://www.addgene.org/105169
Proper Citation: RRID:Addgene_105169
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Clontech = pTRE-T2; Backbone Size:5041; Vector Backbone:pTRE-T2-mIR-Hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A synthetic DNA containing the IRES element was cloned into EcoRI/Xba I site of pTRE-T2-miR-HYGRO vector after removal of the mIR cassette. First digest parental pTRE-T2-miR-HYGRO vector with EcoRI and Xba I to remove the miR cassette. Then the IRES cassette was ligated back to the EcoRI site and Xba I site. The IRES cassette contains the unique EcoRI site followed by unique NotI and Mlu I sites that is followed by the start of the IRES sequence. This cloning site will allow insertion of the 1st cDNA. On the 3’ end of IRES there are unique Spe I and Xba I cloning sites for insertion of the second cDNA into the cassette. Please note- to use the BsaI and XbaI sites for cloning, you must use unmethylated DNA. You will need to isolate the DNA from a methylation deficient bacterial strain.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
