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URL: http://www.addgene.org/104438
Proper Citation: RRID:Addgene_104438
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:30083222
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
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No rating or validation information has been found for pRW004-SM-destination-proUBQ10-Cas9.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
