Apr. 27, 2017

JAX Webinar: CRISPR/Cas9 - Moving from Founder Mice to Phenotyping

CRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relatively simple, many researchers struggle with understanding the complexities associated with the diverse alleles that can be generated, how to deal with mosaicism, and how to move from a single founder mouse to larger cohorts for phenotype characterization. In this webinar, we will discuss the following topics:(1)Understanding the molecular mechanisms that drive the array of possible mutations that can result from a single Cas9/sgRNA targeting strategy (2)A summary of various common targeting strategies to generate indel, knockout, and knockin mutations using CRISPR/Cas9 (3)Selection of founder mice and breeding strategies for mitigating mosaicism in founder mice (4)Breeding strategies to establish a colony and generate cohorts for experimentation.

More information: https://www.jax.org/education-and-learning/education-calendar/webinars/2017/042017/crispr-apr-27

This NIH Funding Opportunity Announcement (FOA) is part of the Stimulating Peripheral Activity to Relieve Conditions (SPARC) Common Fund program. This FOA solicits applications for support of research to gather critical data and answer critical questions on functional peripheral neuroanatomy of organs and reveal the organ function controlled by neural circuits. Organs of interest include those where the peripheral neuroanatomy and functional neurobiology of the organ have been understudied, and which are not the subject of existing SPARC funding under RFA-RM-15-018.

More information:  https://grants.nih.gov/grants/guide/rfa-files/RFA-RM-17-003.html

Apr. 5 , 2017 

Funding Opportunity Application Due Date: Development of Highly Innovative Tools and Technology for Analysis of Single Cells (SBIR) (R43/R44)

This funding opportunity announcement (FOA) encourages Small Business Innovation Research (SBIR) grant applications from small business concerns (SBCs) to develop and validate next-generation single cell analysis technologies and tools. The purpose is to foster the commercialization of innovative single cell analysis technologies for their broad use in biomedical research. The novel single-cell analysis technologies will aid in obtaining a fine-grained and dynamic view of heterogeneous cellular states and intercellular interactions, thereby providing new mechanistic insight into biological processes in health and disease. Applications should define the current state of technologies and tools as a benchmark against which the new approach(es) will be measured. The new approach(es) should provide substantially improved performance in sensitivity, selectivity, spatiotemporal resolution, scalability, multiplexing capability, or non-destructive analysis of molecular or functional measures of single cells.

More information: https://grants.nih.gov/grants/guide/pa-files/PA-17-147.html

Apr. 5, 2017 

Funding Opportunity Application Due Date: Development of Highly Innovative Tools and Technology for Analysis of Single Cells (STTR) (R41/R42)

This funding opportunity announcement (FOA) encourages Small Business Technology Transfer Research (STTR) grant applications from small business concerns (SBCs) to develop and validate next-generation single cell analysis technologies and tools. The purpose is to foster the commercialization of innovative single cell analysis technologies for their broad use in biomedical research. The novel single-cell analysis technologies will aid in obtaining a fine-grained and dynamic view of heterogeneous cellular states and intercellular interactions, thereby providing new mechanistic insight into biological processes in health and disease. Applications should define the current state of technologies and tools as a benchmark against which the new approach(es) will be measured. The new approach(es) should provide substantially improved performance in sensitivity, selectivity, spatiotemporal resolution, scalability, multiplexing capability, or non-destructive analysis of molecular or functional measures of single cells.

More information: https://grants.nih.gov/grants/guide/pa-files/PA-17-148.html

Apr. 17, 2017 

Funding Opportunity Application Due Date: The Chan Zuckerberg Initiative Pilot Projects for a Human Cell Atlas 

The goal of the International Human Cell Atlas project is to create a reference atlas of all cells in the healthy human body as a resource for studies of health and disease. As part of this effort, the Chan Zuckerberg Initiative seeks applications to support the Human Cell Atlas community’s convergence around standards, protocols, and best practices for the creation of a freely and openly available reference database of healthy human cells and tissues. We expect the reference Cell Atlas to ultimately include a variety of data types, including single-cell RNA sequencing, in situ hybridization imaging, proteomics, metabolomics, epigenetic information, low- and high-resolution images of biological samples, and more. For the initial phases of the project, we are especially interested in groups that can help evaluate protocols and best practices for the collection of single-cell molecular data, ideally by directly comparing multiple approaches to tissue preparation, data generation, or data analysis.

More information: https://chanzuckerberg.com/initiatives/rfa