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The interaction between the immune system and endocrine cells in the pancreas is crucial for the initiation and progression of type 1 diabetes (T1D). Imaging mass cytometry (IMC) enables multiplexed assessment of the abundance and localization of more than 30 proteins on the same tissue section at 1-μm resolution. Herein, we have developed a panel of 33 antibodies that allows for the quantification of key cell types including pancreatic exocrine cells, islet cells, immune cells, and stromal components. We employed this panel to analyze 12 pancreata obtained from donors with clinically diagnosed T1D and 6 pancreata from non-diabetic controls. In the pancreata from donors with T1D, we simultaneously visualized significant alterations in islet architecture, endocrine cell composition, and immune cell presentation. Indeed, we demonstrate the utility of IMC to investigate complex events on the cellular level that will provide new insights on the pathophysiology of T1D.
Pubmed ID: 30713110 RIS Download
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Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsSoftware tool to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. It counts cells and also measures the size, shape, intensity and texture of every cell (and every labeled subcellular compartment) in every image. It was designed for high throughput screening but can perform automated image analysis for images from time-lapse movies and low-throughput experiments. CellProfiler has an increasing number of algorithms to identify and measure properties of neuronal cell types.
View all literature mentionsA cloud-based platform which allows users to analyze, visualize, and archive multiparamter cytometry data for single-cell biology. Multiple single-cell data sets can be analyzed and visualized simultaneously with a variety of graphics, inlcuding Sunburst, SPADE, and viSNE. Users can store and back up related data such as protocols, microscopy images, and presentations and collaborate and share analysis and data sets with other Cytobank users. Cytobank also provides a variety of services and training sessions to assist with experiment workflow.
View all literature mentionsA collaborative research project that supports nPOD approved diabetes investigators by freely providing rare and difficult-to-obtain tissues from type 1 and type 2 diabetes donors. Interested researchers are encouraged to apply to obtain nPOD tissues, or to request access to analyze cases in the nPOD Online Pathology site. Interested donors can contact nPOD directly for more information.
View all literature mentionsThis polyclonal targets GHRL
View all literature mentionsThis polyclonal targets Human Pancreatic Polypeptide
View all literature mentionsThis monoclonal targets HLA DR antibody [TAL 1B5]
View all literature mentionsThis polyclonal targets NKX6-1
View all literature mentionsThis polyclonal targets Human CD99
View all literature mentionsThis polyclonal targets NCAM1/CD56
View all literature mentionsThis polyclonal targets Somatostatin
View all literature mentionsThis polyclonal targets Human PDX-1/IPF1 Affinity Purified Ab
View all literature mentionsThis unknown targets Carbonic Anhydrase II Antibody
View all literature mentionsThis monoclonal targets Proinsulin
View all literature mentionsThis unknown targets glucagon
View all literature mentionsNetwork helps to organize and support collaborative research related to loss of functional beta cell mass in Type 1 Diabetes (T1D). Project consists of four independent research initiatives: Consortium on Beta Cell Death and Survival (CBDS), Consortium on Human Islet Biomimetics (CHIB), Consortium on Modeling Autoimmune Interactions (CMAI), Consortium on Targeting and Regeneration (CTAR), and Human Pancreas Analysis Program (HPAP).
View all literature mentionsThis polyclonal targets GHRL
View all literature mentionsNetwork helps to organize and support collaborative research related to loss of functional beta cell mass in Type 1 Diabetes (T1D). Project consists of four independent research initiatives: Consortium on Beta Cell Death and Survival (CBDS), Consortium on Human Islet Biomimetics (CHIB), Consortium on Modeling Autoimmune Interactions (CMAI), Consortium on Targeting and Regeneration (CTAR), and Human Pancreas Analysis Program (HPAP).
View all literature mentionsThis polyclonal targets Human CD99
View all literature mentionsThis polyclonal targets Somatostatin
View all literature mentionsThis polyclonal targets NKX6-1
View all literature mentionsThis polyclonal targets NCAM1/CD56
View all literature mentionsThis polyclonal targets Human Pancreatic Polypeptide
View all literature mentionsThis monoclonal targets HLA DR antibody [TAL 1B5]
View all literature mentionsThis polyclonal targets Human PDX-1/IPF1 Affinity Purified Ab
View all literature mentionsThis unknown targets glucagon
View all literature mentionsThis monoclonal targets Proinsulin
View all literature mentionsThis unknown targets Carbonic Anhydrase II Antibody
View all literature mentions
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant 2U24DK097771-09.
Email: info_at_ dknet.org
